Characterization of microsatellite loci for the detection of temporal genetic shifts within a single cohort of the brown demoiselle,Neopomacentrus filamentosus

Neopomacentrus filamentosus, a common damselfish on the Indo–Australian archipelago, undergoes significant shifts in size and mitochondrial genetic structure upon larval settlement and metamorphosis to juvenile stages. We characterized five polymorphic microsatellite loci in order to study temporal genetic shifts within a single generation of N. filamentosus sampled first as larval settlers then again as demersal juvenile recruits. All loci were extremely polymorphic and exhibited high levels of heterozygosity. While all loci from the larval samples conformed to Hardy – Weinberg expectations, significant heterozygote deficiencies were seen in two loci in the juvenile samples, likely due to extreme size‐selective mortality imposed post‐settlement.     

Natural history of model organisms: The secret (group) life of Drosophila melanogaster larvae and why it matters to developmental ecology

1. Model organisms such as Drosophila melanogaster have been key tools for advancing our fundamental and applied knowledge in biological and biomedical sciences. However, model organisms have become intertwined with the idea of controlled and stable laboratory environments, and their natural history has been overlooked.
2. In holometabolous insects, lack of natural history information on larval ecology has precluded major advances in the field of developmental ecology, especially in terms of manipulations of population density early in life (i.e., larval density). This is because of relativistic and to some extent, arbitrary methodologies employed to manipulate larval densities in laboratory studies. As a result, these methodologies render comparisons between species impossible, precluding our understanding of macroevolutionary responses to population densities during development that can be derived from comparative studies.
3. We recently proposed a new conceptual framework to address this issue, and here, we provide the first natural history investigation of Drosophila melanogaster larval density under such framework. First, we characterized the distribution of larval densities in a wild population of D. melanogaster using rotting apples as breeding substrate in a suburban area in Sweden.
4. Next, we compiled the commonly used methodologies for manipulating larval densities in laboratory studies from the literature and found that the majority of laboratory studies identified did not manipulate larval densities below or above the densities observed in nature, suggesting that we have yet to study true life history and physiological responses to low and high population densities during D. melanogaster development.
5. This is, to our knowledge, the first direct natural history account of larval density in nature for this model organism. Our study paves the way for a more integrated view of organismal biology which re‐incorporates natural history of model organisms into hypothesis‐driven research in developmental ecology.

     

An inexpensive photosynthetic irradiance sensor for ecological field studies

The design and characteristics of inexpensive and simply constructed equal-energy response photosynthetic irradiance sensors is described for use particularly where several cells are required in comparative ecological studies either above or below water. The dimensions of the sensors can be changed proportionally to suit different applications or components. The response of the sensor to irradiance at varying angles corresponds very closely to that required by the cosine law. The sensor is comparatively insensitive to other environmental variables in field use and gave a stable output; the long term drift was proportional to electrical output but in continuous use, drift is regular and could reach -0.08 year^-1 of the total. The spectral range and cosine response is discussed in comparison to other more expensive (x 5–10) commercially available, sensors and to local standards.     

Correspondence between Scientific and Traditional Ecological Knowledge: Rain Forest Classification by the Non-Indigenous Ribereños in Peruvian Amazonia

Traditional ecological knowledge (TEK) is a potential source of ecological information. Typically TEK has been documented at the species level, but habitat data would be equally valuable for conservation applications. We compared the TEK forest type classification of ribereños, the non-indigenous rural peasantry of Peruvian Amazonia, to a floristic classification produced using systematically collected botanical data. Indicator species analysis of pteridophytes in 300 plots detected two forest types on non-flooded tierra firme, each associated with distinct soil texture and fertility, and one forest type in areas subject to flooding. Nine TEK forest types were represented in the same set of plots. Each TEK forest type was consistently (>82%) associated with one of the three floristic classes and there were also clear parallels in the ecological characterizations of the forest types. Ribereños demonstrated clear preferences for certain forest types when selecting sites for slash-and-burn agriculture and hunting. Our results indicate that the non-tribal inhabitants of Amazonia possess valuable TEK that could be used in biodiversity inventories and wildlife management and conservation for characterizing primary rain forest habitats in Amazonia.     

Advancing understanding of the complex nature of urban systems

Cities and urbanized regions are complex, dynamic, and highly integrated systems linking social, ecological, and technical infrastructure domains in ways that create deep challenges for good governance, policymaking, and planning. The combination of impacts from climate change in cities, air pollution, rapid population growth, multiple sources of development pressure and overall urban system complexity make it difficult for decision-makers to develop and guide development trajectories along more livable, equitable, and at the same time, more resilient pathways. Advancing urban sustainability and resilience agendas requires expanding the scope of inter- and trans-disciplinarity approaches, moving beyond the historically separate social–ecological and socio-technical approaches to jointly study social–ecological–technical infrastructure systems in cities. We take urban complexity as a given and suggest that in both research and practice we need to better capture and understand feedbacks, interdependencies, and non-linearities which create uncertainties and challenge the efficacy of governance practices to achieve normative goals for society. Here, we explore new methods, tools, and approaches to advance our understanding of urban system complexity through a series of journal special issue articles that examine urban structure–function relationships, urban sustainability transitions, green space availability, social–ecological memory, functional traits, and urban land use scenarios.     

Analysis of temporal change in delivery of ecosystem services over 20 years at long term monitoring sites of the UK Environmental Change Network

The drivers and pressures experienced by farmland, forestry and upland sites in the UK Environmental Change Network (ECN) over the last 20 years are reported through the lens of recognised approaches to the assessment of ecosystem service delivery. Temporal trends in ecosystem service delivery were examined using two methods: qualitative narratives and quantitative scoring of ecosystem service delivery according to land cover. While all sites included in this study are within the same national governance unit (i.e. UK), individual local management decisions were the main agents driving change, influenced by EU and national policies. Gradual change in focus from provisioning to cultural ecosystem services was a persistent trend across most sites, and apparent in both methods. There was generally no net loss in regulating services at the sites. The two methods were subjective but as data were not available for the breadth of ecosystem services present at the sites between 1993 and 2012, it was concluded that it is more informative for holistic assessments to draw on qualitative expert opinion than to ignore less quantifiable services such as many of the cultural services.